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Brand Name | Yacoo | Model Number | E0018 | Place of Origin | China |
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99% CAS NO 150-25-4 Biological Buffers BICINE N,N-Bis 2-Hydroxyethyl Glycine
Quick Detail:
Name | BICINE | Appearance | White crystalline powder |
Assay,% | 99.99 | Solubility(0.1 M aq.) | Clear, colorless solution |
Loss on drying,% | 0.18 | pH(1% aq.) | 4.89 |
Chloride ion,% | 0.08 | Ultraviolet absorbance(0.1 M aq.) | 0.024 |
Shelf life | two years | Ultraviolet absorbance(0.1 M aq.) | 0.020 |
Description:
> Formula: C6H13NO4
> Weight: 163.1717
> MP/BP:190-192℃(MP)
> Storage: room temperature
> HS:2922499990
> Safety Description: S24/25
Applications:Biological buffer
Synonyms:
DI(HYDROXYETHYL)GLYCINE;DIETHYLOLGLYCINE;DIETHANOLGLYCINE;[BIS-(2-HYDROXY-ETHYL)-AMINO]-ACETIC ACID;BICINE;BICINE BUFFER;LABOTEST-BB LT00452695;n,n-bis(2-hydroxyethyl)-glycin;Diethylolglycine; N,N-Bis(2-hydroxyethyl)glycine; BICINE = N,N-Bis(2-hydroxyethyl)glycine; Bicine, Molecular Biology Grade N,N-Bis(2-hydroxyethyl)glycine, Molecular Biology Grade; Bicine, ULTROL Grade; BICINE buffer Solution; BICINE, [N,N-Bis(2-hydroxyethyl)glycine]; N,N-Di(2-hydroxyethyl)glycine; N,N-Di(2-hydroxyethyl)-glycine; N,N-dihydroxyethylglycine
Related categories:Biochemistry;Good\'s Buffers;Buffer
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Determination of melting point
Instrument:
Micro melting point apparatus WRS-2A, Melting point tubes
Determination of step:
Open switch, stable 20min.
First set the starting temperature than the melting point of the low 10 ℃, heating rate 2 ℃ / min.
The sample was grind as fine as possible and load in a clean, dry melting point pipe ,until the sample was compressed into about 1cm high .When the actual temperature reaches the set temperature and stabilized . Inserted into the capillary with the sample, the simultaneous determination of three.
The determination of PH
Weigh a certain amount of samples into test tube, add a certain amount of water, shake and dissolve, determination of the pH used have been calibrated pH meter.
The determination of UV absorbance
A certain amount of the sample was weighed to the nearest 0.0001g, into 10ml volumetric flask, after constant volume with pure water, at a specific wavelength by 2cm cuvette was measured by UV spectrophotometer.
The determination of Loss on drying
Weigh a sample of approximately 2g (weighing accuracy 0.0001g),Record the weight of m,The sample was placed in a Petri dish constant weight (weight recorded as m0), the tile, the thickness of not more than 5mm, into the oven (As next dish cover removed) drying at 105-110 ℃ 3 hours.When removing, make sure the dish cover,be placed in a desiccator to cool, then weighed and the weight (including surface dish) m1.
Loss on drying in accordance with the following formula:
Where:
m-- sample, g
m0-- dish, g
m1-- After drying the quality of the sample and the dish, g
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