IgG binding regions , Streptococcus Recombinant protein G Sepharose FF

Delivery Time:Depand on stock, without stock need 4 weeks for produce.
Certification:ISO9001:2008

IgG binding regions , Streptococcus Recombinant protein G Sepharose FF for Protein G agarose beads , IgG purification

Product Name:   Recombinant Protein G Conjugated to agarose Resin
Catalog Number:  NRPB02L, NRPB02S (prepacked column)
Packing details:   10 ml, 100 ml, 1L, 1 ml(prepacked column), 2 ml(prepacked column), 5ml(prepacked column), 10ml(prepacked column)
Storage condition:   4oC

Company Group:                                                                                                                      Hangzhou NeuroPeptide Biological Science and Technology Incorporation, Ltd. (NUPTEC----Sales Department)

Add:   Room 11-08, HuaRui Building, No.66, Jianshe Road 1st, Xiaoshan,

          Hangzhou, Zhejiang, China 311202

Hangzhou NUPTEC Rising Bioproducts Incorporation, Ltd. ( NUPTEC----GMP Manufactory)

Add:   600 21st Boulevard, Xiasha Economy and Technology District,

          Hangzhou, China 310018

Introduction:

Protein G is a bacterial cell wall protein expressing at the cell surface of some group C and group G Streptococcal strains. Protein G binds specifically to Fc regions of many mammalian immunoglobulins and is commonly used as affinity adsorbent to purify immunoglobulins (antibodies) and immunoglobulin subtypes from serum, hybridoma ascites, tissue culture supernatants and other biological fluids.

Column characteristics:

Support	6% highly cross-linked spherical agarose
Ligand	Recombinant protein G
Ligand Density	4~5 mg/ml
Particle Size	45~165 μm
Maximum Flow Rate	300 cm/h
Recommended Linear Flow Rate	50~150 cm/h
pH Limits	pH 3~9
Maximum Operating pressure	0.3 MPa
Capacity	≥50mg/ml rabbit IgG or ≥50mg/ml human IgG
Storage	4 oC to 8 oC in 20% ethanol

Some typical binding and elution conditions with proteinG agarose:

Species	Antibody Class	Protein G binding	pH value ofbinding buffer	pH value of elution buffer
Human	IgG1	+++	6.0~7.0	3.5~4.5
IgG2	+++	6.0~7.0	3.5~4.5
IgG3	+++	8.0~9.0	≤7.0
IgG4	+++	7.0~8.0	2.5~4.5
Cow	IgG2	+++	7.0~8.0	2.0
Goat	IgG2	+++	7.0~8.0	5.8
Mouse	IgG1	++	8.0~9.0	5.5~7.5
IgG2a	+++	7.0~8.0	4.5~5.5
IgG2b	+++	7.0	3.5~4.5
IgG3	+++	7.0	4.0~7.0
Rat	IgG1	+	≥9.0	7.0~8.0
IgG2a	+++	≥9.0	≤8.0
IgG2b	+	≥9.0	≤8.0
IgG2c	++	8.0~9.0	3.0~4.0
Rabbit	IgG	+++	7.4	2.7~4.0

“+” represents the binding strength

Performing a separation:

Binding buffer: 20 mM phosphate buffered saline, pH 7.4

Elution buffer: 100 mM glycine-HCl buffer, pH 2.7

Neutralization buffer: 1M Tris-HCl, pH 9.0

1)      Wash the prepacked 1 ml column with 5~10 column volumes of distilled water to remove 20% ethanol.

2)      Equilibrate the column with 5~10 column volumes of binding buffer.

3)      Dilute 5 ml rabbit serum with binding buffer to 50 ml. Filtrate the diluted serum through a 0.45 レm filter and load the sample.

4)      Wash with 5~10 column volumes of binding buffer.

5)      Elute with 5 column volumes of elution buffer and neutralize collect fractions with neutralization buffer.

6)      After each separation cycle, regenerate the resin by washing with approximately 3~5 column volumes of 0.1 M citrate buffer (pH 2.7).

7)       Confirm the purity of the collected antibody by SDS-PAGE analysis (Figure 1, >95% purity).